The reference range of hemolysis on sucrose hemolysis testing (screening and confirmatory tests) is listed below.
Sucrose hemolysis test (screening)
See the list below:
No hemolysis visible – Negative result
Hemolysis present – Positive result
Sucrose hemolysis test (confirmatory)
Hemolysis findings in supernate are as follows:
< 5% – Inconsequential or negative
6-10% – Questionable or borderline
>10% – Positive
The reference range does not vary with age, gender, race, or ethnicity. Note that the percentage of hemolysis on sucrose hemolysis test can vary based on the temperature at which the test is performed, the type of blood used (defibrinated or whole blood), and recent multiple blood transfusions that can dilute the percentage of paroxysmal nocturnal hemoglobinuria (PNH) cells. Therefore, the test must be run at an appropriate temperature, with appropriated blood type and an adequate history of recent blood transfusions to avoid false positives and negative results, respectively.